Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Electron Microscopy Sciences Pipette Tip Universal, 5 - 200 mcL
Pipette tips are low binding to ensure accuracy and precision. The low binding surface is the result of a manufacturing process that binds a perfluorooctyl polymer to polypropylene resin–resulting in an exposed trifluoromethyl surface. This surface reduces the surface energy of liquid and repels any substance so sample retention and binding are minimized.
Not detectable/Non-reactive.
Extracted only by a fluorinated solvent.
Autoclaving will not compromise performance.
Available in convenient hinged racked package.
Tips and racks are fully autoclavable. Natural tips feature reference marks at 10 ul, 50 ul, and 100 u. Low binding surface.
Range: 5 - 200 µl
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Molecular Devices Filter Lens, 550 nm, EMax and VMax Compatible
Replacement optical filter lens with a center wavelength of 550 nm for use in microplate readers. Designed as a field-replaceable spare to restore or modify detection for assays that require a 550 nm bandpass. Supplied as a single unit for straightforward replacement.
- Center wavelength 550 nm.
- Compatible with EMax and VMax microplate readers.
- Single replacement filter, sold each.
- Suitable for absorbance and fluorescence measurements requiring a 550 nm bandpass.
Molecular Devices Emission Filter, 650 nm, 40 nm, FilterMax
Replacement fluorescence emission filter with a 650 nm center wavelength and 40 nm bandwidth, designed for use in Molecular Devices FilterMax F-series multi-mode microplate readers to collect far-red fluorescence emission.
- Center wavelength 650 nm.
- Bandwidth 40 nm for selective far-red detection.
- Designed for installation in filter slide assemblies.
- Enables assays requiring far-red emission collection with reduced background.
- Replacement part for multi-mode microplate reader fluorescence modules.
Ace Glass, Inc. Adapter, 0-10 Hi-Vac valve, no.15 O-ring joint to #15Ace-Thred, No-Air
ADAPTER VAC 0-10VLV NO15-NO15
Molecular Devices Absorbance Filter, 600 nm, 8 nm Bandwidth
The Molecular Devices absorbance bandpass filter centered at 600 nm with an 8 nm full width at half maximum (FWHM) bandwidth is a replacement optical filter for compatible microplate readers. It enables narrow-band absorbance measurements for endpoint and kinetic assays and installs as an interchangeable filter slide.
- Enables narrow-band absorbance measurements at 600 nm.
- 8 nm full width at half maximum bandpass for spectral selectivity.
- Designed for installation as an interchangeable filter slide in compatible microplate readers.
- Suitable for quantitative absorbance assays and endpoint or kinetic measurements.
- Replacement spare for instruments that accept interchangeable absorbance filters.
Molecular Devices Fluorescence Emission Filter, 660 nm, 32 nm Bandwidth
This emission filter is centered at 660 nm with a 32 nm full bandwidth and is intended for use in filter-based fluorescence microplate readers to isolate red emission wavelengths for assays using long-wavelength fluorophores.
- Center wavelength: 660 nm.
- Bandwidth (full width at half maximum): 32 nm.
- Product type: fluorescence emission filter for microplate readers.
- Application: isolates red emission for assays using red fluorophores and dyes.
- Benefit: improves spectral selectivity and reduces signal crosstalk between channels.
Molecular Devices Fluorescence Polarization Filter, 700 nm, Excitation
Molecular Devices 700 nm fluorescence polarization filter designed for fluorescence polarization (FP) microplate assays and near-infrared fluorescence detection. It provides excitation and detection at 700 nm for compatible multi-mode microplate readers.
- Provides excitation and detection centered at 700 nm for fluorescence polarization assays.
- Optimized for fluorescence polarization measurements in microplate formats.
- Enables near-infrared fluorescence detection to reduce background and improve signal-to-noise.
- Designed for easy installation in compatible filter holders and cartridges.
- Suitable for use with multi-mode microplate readers that support user-installable filters.
Molecular Devices Excitation Filter, 585 nm, 10 nm Bandwidth
This excitation filter provides narrowband fluorescence excitation centered at 585 nm with a 10 nm full width, for use in compatible multi-mode microplate readers.
- Center wavelength 585 nm.
- Bandwidth 10 nm full width.
- Excitation filter type suitable for narrowband fluorescence assays.
- Designed for installation in standard filter slide assemblies.
Molecular Devices Multi-Mode Microplate Reader, Five-Mode, HTRF Compatible, 6-384 Wells
The SpectraMax iD5 is a compact, five-mode multi-mode microplate reader designed for flexible bench-top detection of absorbance, fluorescence, luminescence, time-resolved fluorescence, and fluorescence polarization assays. The reader supports optional modules for HTRF and TR-FRET, bottom-read luminescence, and injector-based flash assays, and accepts 6-384 well plates.
- Five built-in detection modes for absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.
- Expandable detection for HTRF, TR-FRET, BRET, and bottom-read luminescence with optional modules.
- Supports 6-384 well plate formats for endpoint and kinetic assays.
- Xenon flash light source with PMT and photodiode detectors for broad wavelength coverage.
- Temperature control to support assays up to 66°C and multiple shaking modes for assay mixing.
- Robotic compatibility for high-throughput automation workflows.
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Molecular Devices Excitation Filter, 540 nm, 25 nm Bandwidth, Bandpass
Molecular Devices FilterMax fluorescence excitation bandpass filter centered at 540 nm with a 25 nm full width, designed for targeted excitation in fluorescence microplate assays. It reduces out-of-band light to improve assay signal-to-noise and installs into compatible filter slide slots for rapid configuration.
- Provides 540 nm excitation with 25 nm bandwidth.
- Bandpass design reduces background and improves signal specificity.
- Optimized for microplate-based fluorescence assays.
- Fits into compatible filter slide slots for quick installation.