Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human & Mouse LC3B II Detection Kit, 500 Assay Points
HTRF Human & Mouse LC3B II Detection Kit, 500 Assay Points
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Flux, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Chemglass Life Sciences Dual Chamber Only, Vacuum Trap
Dual Chamber Only, Vacuum Trap. Replacement part for CG42501 dual chamber vacuum trap.
Revvity Health Sciences Inc HTRF AAV8 Detection Kit, 10,000 Assay Points
HTRF AAV8 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK3 Detection Kit, 10,000 Assay Points
HTRF Human Total JAK3 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total IKZF3 Detection Kit, 10,000 Assay Points
HTRF Human Total IKZF3 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total NRF2 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total NRF2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total HPK1 Detection Kit, 10,000 Assay Points
HTRF Human Total HPK1 Detection Kit, 10,000 Assay Points
Molecular Devices Luminescence Filter, 410 nm, BW 80 nm
This Molecular Devices luminescence emission filter (with holder) provides a 410 nm center wavelength with an 80 nm bandwidth for luminescence detection in microplate assays. It is listed as an accessory for SpectraMax iD series multi-mode microplate readers and is commonly used as a donor emission filter in assays such as BRET2.
- Provides a 410 nm center wavelength with an 80 nm bandwidth
- Includes holder for secure installation in filter slot
- Optimized for luminescence emission detection
- Used as donor emission filter in luminescence assays
- Compatible with multi-mode microplate reader filter systems
Molecular Devices Fluorescence Excitation Filter, 525 nm, 45 nm Bandwidth
The Molecular Devices 525 nm fluorescence excitation filter (6590-0052) is a 45 nm bandpass excitation filter designed for fluorescence assays requiring green-range illumination. It is intended for use with multi-mode microplate readers that accept filter slide carriers and provides selective excitation centered at 525 nm.
- Filter type: fluorescence excitation filter.
- Center wavelength: 525 nm.
- Bandwidth: 45 nm.
- Mounting format: filter slide compatible with multi-filter slide carriers.
- Application: optimized for green-range fluorescence assays.
Molecular Devices Fluorescence Filter, 425 nm, 40 nm, With Holder
Molecular Devices fluorescence filter with holder provides a 425 nm central wavelength and 40 nm bandwidth for selective optical isolation in fluorescence, luminescence, and time-resolved fluorescence (TRF) assays. The filter comes mounted in a holder for integration into compatible multi-mode microplate readers and imaging modules.
- Designed for fluorescence, luminescence, and TRF assays.
- 425 nm central wavelength with 40 nm bandwidth.
- Supplied in a holder for instrument mounting.
- Reduces background signal and improves specificity.
- Suitable for integration with multi-mode microplate readers and imaging systems.
Molecular Devices Fluorescence Filter, 625 nm, 35 nm BW
Replacement fluorescence emission filter supplied with a mounting holder for use in compatible Molecular Devices microplate readers. The filter provides a red-region emission band centered at 625 nm with a 35 nm full bandwidth, suitable for fluorescence intensity and time-resolved fluorescence assays.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Includes mounting holder for slide insertion.
- Optimized for red-emission fluorescence detection.
- Intended for use in microplate readers that accept plug-in filters.
Molecular Devices Fluorescence Excitation Filter, 370 nm, Time-Resolved, Single Unit
Molecular Devices 370 nm time-resolved fluorescence filter provides excitation at 370 nm for time-resolved fluorescence assays. It is supplied as a single replacement optical filter for compatible fluorescence instruments.
- Provides excitation centered at 370 nm.
- Designed for time-resolved fluorescence measurements.
- Supplied as a single replacement optical filter.
- Intended for use in compatible fluorescence detection instruments.
- Helps improve excitation specificity for time-resolved assays.
Molecular Devices Fluorescence Filter, 590 nm, 20 nm bandwidth, Holder
Molecular Devices fluorescence emission bandpass filter with holder designed for narrow emission detection at 590 nm. The filter provides a 20 nm full width passband and installs in a compatible filter holder for microplate reader fluorescence assays.
- Provides a narrow emission passband centered at 590 nm.
- 20 nm full width bandwidth for selective signal detection.
- Supplied mounted in a holder for straightforward instrument installation.
- Optimized for fluorescence emission measurements to improve assay specificity.
- Reduces spectral crosstalk for improved signal-to-noise ratio.
